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Method Development in Solid Phase Extraction using Non-polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples
TechNote #101 – This Chemistry Data Sheet includes specific information on the extraction of analytes from aqueous samples (pages 1-3) and a general discussion on the range of ISOLUTE non-polar sorbents (page 4).
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Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Sorbents for the Extraction of Non-Aqueous Samples
TechNote #102 – This Chemistry Data Sheet includes specific information on the use of polar ISOLUTE sorbents for the extraction of polar analytes from non-aqueous samples (page 1-2), and a general discussion on polar sorbents (page 3). |
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Method Development in Solid Phase Extraction using ISOLUTE SAX and ISOLUTE PE-AX Columns for the Extraction of Aqueous Samples
TechNote #103 – This technical notes includes specifics on the use of ISOLUTE SAX and PE-AX for the extraction of anionic analytes from acqueous samples (pages 1-3), ordering information (page 4) and a general discussion on anion exchangers (pages 5-6). |
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Method Development in Solid Phase Extraction using ISOLUTE NH2 SPE columns for the Extraction of Aqueous Samples
TechNote #104 – This technical note includes specifics on the use of ISOLUTE NH2 for the extraction of anionic analytes from aqueous samples (pages 1-2), order information (page 3) and a general discussion on anion exchangers (pages 4-5). |
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Method Development in Solid Phase Extraction using ISOLUTE PSA SPE Columns for the Extraction of Aqueous Samples
TechNote #105 – This technical note includes specifics on the use of ISOLUTE PSA for the extraction of anionic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on anion exchangers (pages 4-5). |
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Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX-3 SPE Columns for the Extraction of Aqueous Samples
TechNote #106 – This technical note includes specifics on the use of ISOLUTE SCX and SCX-3 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchangers (pages 4-5). |
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Method Development in Solid Phase Extraction using ISOLUTE SCX-2 SPE Columns for the Extraction of Aqueous Samples
TechNote #107 – This technical note includes, specifics on the use of ISOLUTE SCX-2 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchanger sorbents (pages 4-5). |
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Method Development in Solid Phase Extraction using ISOLUTE CBA SPE Columns for the Extraction of Aqueous Samples
TechNote #108 – This technical note includes specifics on the use of ISOLUTE CBA for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchange sorbents (pages 4-6). |
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Method Development in Solid Phase Extraction using ISOLUTE ENV+ SPE Columns for the Extraction of Aqueous Samples
TechNote #109 – This technical note includes a general discussion on non-polar sorbents (page 1), specifics on the use of ISOLUTE ENV+ for the extraction of polar analytes from aqueous samples (page 2), and ordering information (page 5). |
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SPE Modes of Operation
TechNote #110 – A list of the five typical ways of using SPE. Each one offers a solution to different types of sample preparation challenges. |
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Matrix Solid Phase Dispersion
TechNote #111 – Matrix Solid Phase Dispersion (MSPD) is a sample preparation technique for use with solid sample matrices. Conventional extraction of organic analytes from tissue usually begins with a homogenization step, followed by tedious liquid-liquid extraction procedures, sample clean-up and trace enrichment. A relatively large amount of sample matrix is required, and solvent consumption is high. |
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General approach to the Extraction of Basic Drugs from Biological Fluids using Non-polar ISOLUTE SPE Columns
TechNote #112 – This technical note describes the extraction of basic drugs from biological fluids using non-polar ISOLUTE SPE columns. The extraction of drugs from biological fluids using a purely non-polar retention mechanism can lead to extracts containing a large amount of non-polar co-extracted material, which can interfere with the subsequent analysis. |
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General Approach to the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Confirm HCX Mixed Mode SPE Columns
TechNote #113 – ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug. |
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Solid Phase Extraction using the 96-well Format ISOLUTE-96 Fixed Well Plates and ISOLUTE Array 96-well Plates
TechNote #114 – New approaches to the quantitation of drugs and metabolites in biological fluids have increased the need for high throughput methodologies to avoid the traditional laboratory bottleneck of sample preparation. Solid phase extraction in a 96-well plate format such as ISOLUTE-96 Fixed Well Plates or ISOLUTE Array 96-well Plates is a solution to this problem. |
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Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed-mode SPE Columns and 96-well SPE Plates
TechNote #116 – The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix. |
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The use of ISOLUTE HM-N for rapid sample preparation
TechNote #118 – ISOLUTE HM-N is a modified form of diatomaceous earth that can efficiently absorb aqueous samples. ISOLUTE HM_N is chemically inert and stable in the pH range 1-13. These characteristics make it a versabile material that plays an important role in many sample preparation applications. |
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Method Development in Solid Phase Extraction using ISOLUTE 101 SPE Columns for the Extraction of Aqueous Samples
TechNote #119 – This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3). |
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Sample Preparation by Mixed-mode SPE using ISOLUTE HCX
TechNote #125 – The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract. |
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Sample Preparation by Non-polar SPE using ISOLUTE SPE Sorbents
TechNote #126 – ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent. |
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Sample Preparation by Mixed-mode using ISOLUTE HAX
TechNote #127 – ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract. |
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Generic Method for the Extraction of Quaternary Amines and Polybasic Drugs from Biological Fluids using ISOLUTE HCX-Q Mixed-Mode SPE Columns and 96-well Plates
TechNote #129 – The use of ISOLUTE HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The ISOLUTE HCX-Q sorbent utilizes a combination of weak cation exchange and C8 non-polar retention mechanisms. |
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Sample Preparation by Protein Precipitation Using the ISOLUTE Protein Precipitation Plate
TechNote #130 – ISOLUTE PPT+ Protein Precipitation Plates have been designed to overcome the problems of the first generation 96-well filterplates. The functionalized bottom frit holds up organic solvents, in particular acetonitrile, allowing the precipitating solvent to be dispensed into each well prior to sample addition. |
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EVOLUTE ABN for Extraction of Drugs from Biological Fluids
TechNote #131 – EVOLUTE Sample Preparation Products are a new generation of advanced polymeric solid phase extraction sorbents for the high throughput extraction of drugs from biological fluids. Designed to maximize analyte recovery, while reducing the concentration of matrix components in the final extract, EVOLUTE products provide improved extract cleanliness that minimizes ion suppression effects during LC-MS/MS analysis. |
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EVOLUTE ABN SPE Columns for Solid Phase Extraction of Environmental Samples
TechNote #136 – EVOLUTE ABN (Acidic Base Neutral) has been developed for the extraction of acidic, neutral or basic compounds from a variety of aqueous matrices. The polystyrene based polymer is surface modified with well defined hydroxyl-functional oligomers, imparting excellent water-wettability. An optimized combination of non-polar (hydrophobic) and polar (hydrophilic) interactions allows efficient extraction of analytes of wide ranging polarities. These characteristics result in a versatile sorbent for extraction of the broad range of analytes encountered in routine environmental analysis. |
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EVOLUTE ABN Columns for Solid Phase Extraction of Forensic and Clinical Samples
TechNote #137 – This Chemistry Data Sheet provides guidelines for the simultaneous extraction of acidic, basic and neutral compounds from biological fluids samples using polymer-based non-polar SPE. The generic method is on page 1, with processing and method optimization guidelines on page 2-3. An example application involving the extraction of diuretics from urine illustrates the versatility of EVOLUTE ABN for the extraction of a wide range of analytes (see Appendix). |
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EVOLUTE ABN Columns for Solid Phase Extraction of Aqueous Samples
TechNote #138 – This Chemistry Data Sheet provides guidelines for the simultaneous extraction of acidic, basic and neutral compounds from aqueous samples or aqueous sample extracts using polymer-based non-polar SPE. The generic method is on page 1, with processing and method optimization guidelines on pages 2-3. An exmaple application showing the trace enrichment of pharmaceuticals in water illustrates the versatility of EVOLUTE ABN for the extraction of a wide range of analytes (see Appendix) from aqueous samples. |
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EVOLUTE® CX Columns for Solid Phase Extraction in Bioanalysis
TechNote #139 – This Chemistry Data Sheet provides guidelines for the extraction of basic drugs from biological fluid samples using resin-based mixed-mode SPE. |
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ISOLUTE SLE+ Supported Liquid Extraction Plates for the Extraction of Drugs from Biological Fluids
TechNote #140 – ISOLUTE SLE+ 200 and 400 mg Supported Liquid Extraction Plates contain a modified form of diatomaceous earth. The support provides an inerg interface between the two liquids, allowing rapid transfer of the drugs into the extraction solvent. |
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EVOLUTE® CX Columns for Solid Phase Extraction of Basic Compounds from Aqueous Samples
TechNote #141 – This Chemistry Data Sheet provides guidelines for the extraction of basic analytes from aqueous samples using resin-based mixed-mode SPE. |
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ISOLUTE® EPH Solid Phase Extraction Columns for the Fractionation of Extractable Petroleum Hydrocarbons from Soil using both Automated and Manual Procedures
TechNote #142 – This Chemistry Data Sheet provides for both the automated and manual fractionation of EPH in soil. |
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EVOLUTE® WCX Columns for Solid Phase Extraction of Quaternary Amines and Strongly Basic Compounds from Aqueous Samples
TechNote #143 – This Chemistry Data Sheet provides guidelines for the extraction of quaternary amines and strongly basic analytes from aqueous samples using EVOLUTE WCX |
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EVOLUTE® WAX Columns and Plates for Solid Phase Extraction of Strongly Acidic Compounds from Aqueous Samples
TechNote #146 – This Chemistry Data Sheet provides guidelines for the extraction of strongly acidic compounds from aqueous samples using EVOLUTE WAX. The procedure is described on pages 1, with processing and optimization guidelines on page 2. An example application showing the extraction of pesticide metabolites from urine illustrates the use of EVOLUTE WAX (see Appendix 1) for clean-up of biological fluid samples. |
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Step by step guide to method development |