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Biotage® Isolera™ ELSD-1080
Evaporative Light-scattering Detection
for flash chromatography
- Natural Products
The Biotage Isolera ELSD (evaporative light-scattering detector) is a universal detector designed for use with Isolera flash purification systems when purifying organic compounds that are undetectable UV or visible light. Flash chromatography with detection and fractionation is now possible when purifying carbohydrates, steroids, lipids, terpenes and other UV-transparent compounds.
- Flexibility – high sensitivity provides superb responses for all compounds, able to detect virtually any compound
- Sub-ambient operation – provides detection capability for highly volatile compounds with operation at temperatures as low as 10 °C up to 80 °C
- Reproducible - below 2% with reliable and accurate results
- Independent temperature controls – for both nebulizer and evaporator provides optimization capability for normalphase solvent systems
- Compact size – requires minimal bench space
- Compatible and portable – operates with other analytical HPLC systems
Organic compounds have different chemical and physical properties and they impact detection, even with an ELSD. The Biotage Isolera ELSD-1080 provides intelligent method design which enables the chemist to independently set nebulizer and evaporator temperatures for a particular compound or compound class. Independent temperature control helps ensure that all compounds are detected.
Figure 1. Separation of 3-Nitroaniline and 1,2:5,6-Di-O-Isopropylidene-a-D-Glucofuranose using an Biotage Isolera UV Flash system with ELS Detection and Biotage SNAP Biotage KP-Sil 25g cartridges. The Glucofuranose is only detected by ELSD.
Incorporated with the independent temperature control is the ability to evaporate solvents at temperatures as low as 10 °C. This is desirable when purifying highly volatile compounds.
How does ELSD work?
Sample eluting from a flash cartridge enters the ELSD where it is mixed with compressed nitrogen to nebulizer the sample (creates small droplets). The nebulizer is heated and begins to evaporate the solvent and the nitrogen pushes the sample into a heating chamber where the remaining solvent is evaporated leaving small sample particles in the nitrogen stream. While migrating through the evaporator, light is shone perpendicular to the “sample flight path” and a sensor measures how much light is coming through. Loss of light transmission is proportional to the amount of compound. This information is then relayed to the Isolera where it is then collected.
||90/120V AC or 220/250V AC, 50/60 Hz 2A max
and data management
|200 mm (8”) x 415 mm (16”) x 450 mm (18”) diagonal touch screen interface
|Dimensions (W x H x D)
||200 mm (8”) x 415 mm (16”) x 450 mm (18”)
||13 kg (28 lb)
||LED 480 nm (Class 1 LED Product)
||Photomultiplier tube digital signal processing
||Evaporator OFF, 10 – 80 °C (1 °C increments) Nebulizer OFF, 25 – 90 °C (1 °C increments)
|Gas Flow rate
||0.9 SLM to 3.25 SLM @ 25 °C with integrated controlled gas shut off valve
|Pressure operating range
||60 – 100 psi (4 – 6.7 bar)
|Eluent flow rate
||0.2 – 5 mL/min
||0 – 5V FSD
||24 bit digital data, 10 or 40 Hz
||Serial (RS232) Remote Start Input Remote A/Z Contact closure TTL
||Graphical vacuum fluorescent display with keypad Ten pre-defined methods
||Gas shut off valve, vapor and leak detection
||Biotage Isolera ELSD-1080
|ELSD-1080 flow splitter kit