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Biotage breaks new ground with its fully integrated mass directed flash systemBiotage and Peptide SynthesisComing soon: Isolera™ Dalton - Flash Chromatography with Integrated Mass IdentificationISOLUTE® Myco SPE columns, a dedicated product for the efficient extraction of mycotoxins from food matricesTLC-to-Step Gradient capability significantly reduces both purification time and solvent useBiotage Releases New Catalog and Technical Guide for Sample Preparation and Evaporation
 

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ISOLUTE® QuEChERS
Quick, Easy, Cheap, Effective, Rugged, and Safe

 Features and Benefits
  • No need to weigh individual components – faster and more consistent
  • Include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples
  • Include clean up tubes for the dispersive solid phase extraction
  • Unique shipping and storage rack
  • Solvent resistant paper labels aid hassle free sample identification
Product Photo

Fast and efficient clean up of complex samples

ISOLUTE QuEChERS contain a mixture of precisely measured chemical compounds as described in the AOAC and EN standard methods for the analysis of pesticide residues from fruit and vegetable samples. Unlike labor intensive liquid-liquid extraction and complicated solid phase extraction methods, QuEChERS combines clean extracts with rapid work flow and ease of use that ensures reproducibility and faster sample preparation. Biotage’s strict quality control during the manufacturing process ensures consistent performance in an already well defined methodology.

The 15 mL extraction and clean-up tubes contain sufficient sorbent to process samples according to prescribed AOAC and EN methodologies and allow for immediate sample mixing and transfer to larger 50mL sample tubes. All QuEChERS products are shipped in a cardboard rack for ease of use and contain full instructions following the EN and AOAC methodologies. 

 

QuEChERS technique involves two stages:
  1. A crude extraction and partitioning step using organic solvent and a salt solution.
  2. Followed by further extraction and clean up using dispersive solid phase extraction.


AOAC 2007.01 and European EN 15662 Methodologies

Biotage® supplies pre-weighed extraction and clean up tubes that conform with AOAC1,2 and the EU3 published methods, including those for waxed and highly pigmented fruit and vegetable samples.

Figure 1 Figure 2
Figure 1 Apple Figure 2 Broccoli

Figure 1 and 2: Pesticide suite was extracted using Biotage QuEChERS products and the relevant methodology (AOAC or EN) for apple (Figure 1 above) and Broccoli (Figure 2 above). In all instances, recoveries were above 75%.


Figure 3 Figure 4
Figure 3 Figure 4
Figure 3 and Figure 4: Pesticide suite was extracted using Biotage QuEChERS products and the relevant methodology (AOAC or EN) for spinach (Figure 3 above) and orange (Figure 4 above). In all instances, recoveries were above 80%.

References

  1. AOAC Official Method 2007.01. Pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate.
  2. Anastassiades, M., Lehotay, S.J., Stajnbaher, D. and Schenk, F.J. (2003) J AOAC International 86, 412-431.  
  3. EN 15662. Foods of plant origin- Determination of pesticide residues using GC-MS and /or LC-MS/MS following acetonitrile extraction/partitioning and clean up by dispersive SPE-QuEChERS method.

Ordering Information

Extraction Tubes
Part Number Description
Tube Pack
Size
MgSO4 Na Acetate Na Citrate Na Citrate
Sesquihydrate
NaCI
Q0010-15V 15 g QuEChERS AOAC 15 mL Extraction Tube 15 mL 25 6 g 1.5 g      
Q0020-15V 10 g QuEChERS EN 15 mL Extraction Tube 15 mL 25 4 g   1 g 0.5 g 1 g
Clean-up Tubes
Part Number Description Tube Pack
Size
PSA MgSO4
(purest)
C18(EC) GCB
Q0030-15V AOAC Fruit and Vegetables Cleanup Tube 15 mL 25 400 mg 1200 mg    
Q0035-15V EN Fruit and Vegetable Cleanup Tube 15 mL 25 150 mg 900 mg    
Q0050-15V AOAC Waxed Fruit and Vegetables Cleanup Tube 15 mL 25 400 mg 1200 mg 400 mg  
Q0060-15V EN Waxed Fruit and Vegetables Cleanup Tube 15 mL 25 150 mg 900 mg 150 mg  
Q0070-15V AOAC Pigmented Fruit and Vegetables Cleanup Tube 15 mL 25 400 mg 1200 mg   400 mg
Q0080-15V EN Pigmented Fruit and Vegetables Cleanup Tube 15 mL 25 150 mg 900 mg   15 mg
Q0090-15V EN Highly Pigmented Fruit and Vegetables Cleanup Tube 15 mL 25 150 mg 900 mg   45 mg
Centrifuge Tubes
Part Number Description Tube Pack Size
Q0000-50V 50 mL Centrifuge Tube with Rack 50 mL 25

 

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